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验证数据展示

  • WB analysis using 86324-1-RR (same clone as 86324-1-PBS)

    WB analysis using 86324-1-RR (same clone as 86324-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86324-1-RR (KRT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 86324-1-RR (same clone as 86324-1-PBS)

    IHC staining of human skin cancer using 86324-1-RR (same clone as 86324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT10 antibody included
  • IHC staining of human skin cancer using 86324-1-RR (same clone as 86324-1-PBS)

    IHC staining of human skin cancer using 86324-1-RR (same clone as 86324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT10 antibody included
  • IF Staining of mouse skin using 86324-1-RR (same clone as 86324-1-PBS)

    IF Staining of mouse skin using 86324-1-RR (same clone as 86324-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 86324-1-RR (same clone as 86324-1-PBS)

    IF Staining of mouse skin using 86324-1-RR (same clone as 86324-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86324-1-PBS in a different storage buffer formulation.

Cytokeratin 10 Recombinant monoclonal antibody, PBS Only

Cytokeratin 10 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86324-1-PBS

产品说明书

CloneNo. 250893H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

KRT10, CK 10, CK10, CK-10, Cytokeratin,Keratin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KRT10 Ready-To-Use IHC Kit. KRT10 primary antibody included.

产品信息

86324-1-PBS targets Cytokeratin 10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 10
别名 KRT10, CK 10, CK10, CK-10, Cytokeratin,Keratin
计算分子量 584 aa, 59 kDa
观测分子量59 kDa
GenBank蛋白编号BC034697
基因名称 Cytokeratin 10
Gene ID (NCBI) 3858
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13645
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 10 (CK-10, K10, KRT10) is a type I (acidic) cytokeratin and part of the intermediate filament protein superfamily. It is expressed in keratinized stratified epithelium, suprabasal cell layers (including the stratum corneum), and differentiated areas of highly differentiated squamous cell carcinomas. CK-10 plays a role in maintaining the structural integrity of epithelial cells and is involved in differentiation, tissue specialization, and epidermal barrier establishment. Mutations in the KRT10 gene are associated with epidermolytic hyperkeratosis, and its expression is linked to the terminal differentiation of epidermal cells . CK-10 is also used as a marker for tissue differentiation and characterization of malignant tumors in surgical pathology.

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