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验证数据展示

  • WB analysis of human saliva using 10823-1-AP (same clone as 10823-1-PBS)

    WB analysis of human saliva using 10823-1-AP (same clone as 10823-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 10823-1-AP (Cystatin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    IF Staining of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10823-1-AP (same clone as 10823-1-PBS)

    IF Staining of HepG2 using 10823-1-AP (same clone as 10823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cystatin B antibody (10823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    IP experiment of MCF-7 using 10823-1-AP (same clone as 10823-1-PBS)

    IP result of anti-Cystatin B (IP:10823-1-AP, 4ug; Detection:10823-1-AP 1:4000) with MCF-7 cells lysate 1320ug. This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 10823-1-AP (same clone as 10823-1-PBS)

    IHC staining of human colon using 10823-1-AP (same clone as 10823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSTB antibody included
  • IHC staining of human stomach using 10823-1-AP (same clone as 10823-1-PBS)

    IHC staining of human stomach using 10823-1-AP (same clone as 10823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10823-1-AP (Cystatin B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSTB antibody included
  • Featured Product
  • KD/KO Validated

Cystatin B Polyclonal antibody, PBS Only

Cystatin B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10823-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CSTB, EPM1, Cystatin-B, CST6, CPI-B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CSTB Ready-To-Use IHC Kit. CSTB primary antibody included.

产品信息

10823-1-PBS targets Cystatin B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1262

Product name: Recombinant human CSTB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-98 aa of BC003370

Sequence: MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cystatin B (stefin B)
别名 CSTB, EPM1, Cystatin-B, CST6, CPI-B
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC003370
基因名称 Cystatin B
Gene ID (NCBI) 1476
RRIDAB_2086100
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04080
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

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