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验证数据展示

  • WB analysis using 20992-1-AP (same clone as 20992-1-PBS)

    WB analysis using 20992-1-AP (same clone as 20992-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 20992-1-AP (same clone as 20992-1-PBS)

    WB analysis of K-562 using 20992-1-AP (same clone as 20992-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 20992-1-AP (same clone as 20992-1-PBS)

    IF Staining of MCF-7 using 20992-1-AP (same clone as 20992-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin T1 antibody (20992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 20992-1-AP (same clone as 20992-1-PBS)

    IHC staining of human ovary cancer using 20992-1-AP (same clone as 20992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20992-1-AP (Cyclin T1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

Cyclin T1 Polyclonal antibody, PBS Only

Cyclin T1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20992-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CCNT1, CCNT, CYCT1, Cyclin-T1, Cyclin-T

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20992-1-PBS targets Cyclin T1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin T1
别名 CCNT1, CCNT, CYCT1, Cyclin-T1, Cyclin-T
计算分子量 81 kDa
观测分子量 81 kDa
GenBank蛋白编号NM_001240
基因名称 Cyclin T1
Gene ID (NCBI) 904
RRIDAB_10950894
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60563
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

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