验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells, K-562 cells, Jurkat cells, HeLa cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human pancreas tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26755-1-AP targets Cyclin D3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat, sheep |
| 免疫原 |
CatNo: Ag25151 Product name: Recombinant human Cyclin D3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 250-292 aa of BC011616 Sequence: ALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin D3 |
| 别名 | CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC011616 |
| 基因名称 | Cyclin D3 |
| Gene ID (NCBI) | 896 |
| ENSEMBL Gene ID | ENSG00000112576 |
| RRID | AB_2756524 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30281 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Cyclin D3 antibody 26755-1-AP | Download protocol |
| IHC protocol for Cyclin D3 antibody 26755-1-AP | Download protocol |
| IP protocol for Cyclin D3 antibody 26755-1-AP | Download protocol |
| WB protocol for Cyclin D3 antibody 26755-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Glucose-responsive, antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesis | ||
Nucleic Acids Res Small nucleolar RNAs promote the restoration of muscle differentiation defects in cells from myotonic dystrophy type 1 | ||
J Exp Clin Cancer Res HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway. | ||
Cell Death Differ The LINC01315-encoded small protein YAPer-ORF competes with PRP4k to hijack YAP signaling to aberrantly promote cell growth |
