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验证数据展示

  • WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85895-3-PBS in a different storage buffer formulation.

  • WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85895-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85895-3-RR

    Affinity and Kinetic Characterization of 85895-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85895-3-RR against Human Cyclin B1 were performed. The affinity constant is 2.70 nM.

Cyclin B1 Recombinant monoclonal antibody, PBS Only

Cyclin B1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85895-3-PBS

产品说明书

CloneNo. 250227C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.

产品信息

85895-3-PBS targets Cyclin B1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29426

Product name: Recombinant human CCNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-140 aa of BC006510

Sequence: MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin B1
别名 CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1
计算分子量 48 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC006510
基因名称 Cyclin B1
Gene ID (NCBI) 891
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14635
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

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