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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85895-3-PBS in a different storage buffer formulation.

  • WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    WB analysis using 85895-3-RR (same clone as 85895-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85895-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85895-3-RR

    Affinity and Kinetic Characterization of 85895-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85895-3-RR against Human Cyclin B1 were performed. The affinity constant is 2.70 nM.

Cyclin B1 Recombinant antibody, PBS Only

Cyclin B1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85895-3-PBS

产品说明书

CloneNo. 250227C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.

产品信息

85895-3-PBS targets Cyclin B1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Cyclin B1 fusion protein Ag29426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin B1
别名 CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1
计算分子量 48 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC006510
基因名称 Cyclin B1
Gene ID (NCBI) 891
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14635
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.