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验证数据展示

  • WB analysis using 55004-1-AP (same clone as 55004-1-PBS)

    WB analysis using 55004-1-AP (same clone as 55004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)

    IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNB1 antibody included
  • IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)

    IHC staining of human skin cancer using 55004-1-AP (same clone as 55004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55004-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CCNB1 antibody included
  • IF Staining of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    IF Staining of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    FC experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Cyclin B1 Polyclonal antibody (55004-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 55004-1-AP (same clone as 55004-1-PBS)

    IF Staining of U2OS using 55004-1-AP (same clone as 55004-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Cyclin B1 antibody (55004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    IP experiment of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    IP result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    WB result of Cyclin B1 antibody (55004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    WB analysis of HeLa using 55004-1-AP (same clone as 55004-1-PBS)

    Non-treated HeLa and thymidine or nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 55004-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cyclin B1 Polyclonal antibody, PBS Only

Cyclin B1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 55004-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.

产品信息

55004-1-PBS targets Cyclin B1 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin B1
别名 CCNB1, G2/mitotic-specific cyclin-B1, G2/mitotic specific cyclin B1, Cyclin B, CCNB
计算分子量 48 kDa
观测分子量 55-60 kDa
GenBank蛋白编号NM_031966
基因名称 Cyclin B1
Gene ID (NCBI) 891
RRIDAB_10859790
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14635
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

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