验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
31992-1-AP targets Cubilin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Cubilin fusion protein Ag35808 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cubilin (intrinsic factor-cobalamin receptor) |
| 别名 | Intrinsic factor-vitamin B12 receptor, Intrinsic factor-cobalamin receptor, Intestinal intrinsic factor receptor, IFCR, CUBN |
| 计算分子量 | 398kDa |
| 观测分子量 | 450 kDa |
| GenBank蛋白编号 | NM_001081 |
| 基因名称 | CUBN |
| Gene ID (NCBI) | 8029 |
| RRID | AB_3670168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O60494 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. Cubilin is an endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake (PMID: 9572993). Cubilin Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1, which also acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex (PMID: 14576052). There are multiple glycosylation sites on Cubilin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cubilin antibody 31992-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |