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验证数据展示

  • IHC staining of mouse brain using 13474-1-AP (same clone as 13474-1-PBS)

    IHC staining of mouse brain using 13474-1-AP (same clone as 13474-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cryptochrome 1/CRY1 antibody included
  • WB analysis using 13474-1-AP (same clone as 13474-1-PBS)

    WB analysis using 13474-1-AP (same clone as 13474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

    FC experiment of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

    IF Staining of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody (13474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cryptochrome 1 Polyclonal antibody, PBS Only

Cryptochrome 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 13474-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CRY1, CRY 1, Cryptochrome-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Cryptochrome 1/CRY1 Ready-To-Use IHC Kit. Cryptochrome 1/CRY1 primary antibody included.

产品信息

13474-1-PBS targets Cryptochrome 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4270

Product name: Recombinant human CRY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 236-586 aa of BC030519

Sequence: RPRMNANSLLASPTGLSPYLRFGCLSCRLFYFKLTDLYKKVKKNSSPPLSLYGQLLWREFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLSCSSFFQQFFHCYCPVGFGRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNAPEGIQKVAKCLIGVNYPKPMVNHAEASRLNIERMKQIYQQLSRYRGLGLLASVPSNPNGNGGFMGYSAENIPGCSSSGSCSQGSGILHYAHGDSQQTHLLKQGRSSMGTGLSGGKRPSQEEDTQSIGPKVQRQSTN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cryptochrome 1 (photolyase-like)
别名 CRY1, CRY 1, Cryptochrome-1
计算分子量 586 aa, 66 kDa
观测分子量60-66 kDa
GenBank蛋白编号BC030519
基因名称 CRY1
Gene ID (NCBI) 1407
RRIDAB_10697652
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16526
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.

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