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Validation Data Gallery

  • Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10594-1-AP (PEX14 antiboody) and CoraLite®647 conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) antibody (SA00014-9) at dilution of 1:150.

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10594-1-AP (PEX14 antiboody) and CoraLite®647 conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) antibody (SA00014-9) at dilution of 1:150.

  • 1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-human BCL2 Polyclonal antibody (12789-1-AP) and CoraLite®647-Conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-human BCL2 Polyclonal antibody (12789-1-AP) and CoraLite®647-Conjugated AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite647-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)

Cat No. SA00014-9

Applications

IF, FC

Conjugate

CoraLite®647

Host

Goat

Reactivity

Rabbit

规格价格库存

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Product Information

Concentration 0.3 mg/mL
Applications IF, FC
Host Species Goat
Species Reactivity Rabbit
Physical State Liquid
Suggested Dilution Range 1:100 – 1:500
Purity The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 5 mg/ml BSA, 0.01% Sodium Azide, pH 7.6.
Preservative0.01% Sodium Azide
Storage Instructions Store at -20°C. Stable for one year after shipment.
FluorophoreCL647 Fluorescent Dye. Amax=642nm, Emax=662nm
Stabilizer5 mg/ml BSA
Absorbance RatioA651/A280 = 4.2
ClonalityPolyclonal

背景介绍

N/A

Publications

Title

Bone Res

Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles

Authors - Weicheng Lu
View Article

Sci Rep

A novel role of peroxiredoxin 2 in diabetic kidney disease progression by activating the classically activated macrophages

Authors - Xia Li
View Article

Food Sci Nutr

Peimine Promotes Skin Wound Repair in Mice by Activating the Notch1 Signaling Pathway in Fibroblasts

Authors - Peng Wu
View Article

Drug Resist Updat

Exosome-derived lnc-FAM72D-3 promotes lenvatinib resistance by remodeling hepatocellular carcinoma cytoskeleton via MBNL1/FAK axis

Authors - Mingbo Cao
View Article

Brain Res Bull

Synaptic vesicle protein 2A regulates mossy fiber sprouting in a drug-resistant epilepsy rat model via laminin α5/integrin β1

Authors - Yongfei Shi
View Article

Signal Transduct Target Ther

A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics

Authors - Zhiheng Rao
View Article
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