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验证数据展示

  • WB analysis using 84101-5-RR

    WB analysis using 84101-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84101-5-RR (Complement factor B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84101-5-RR

    IHC staining of mouse liver using 84101-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84101-5-RR

    IHC staining of mouse liver using 84101-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84101-5-RR

    IF Staining of HepG2 using 84101-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor B antibody (84101-5-RR, Clone: 241141A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 84101-5-RR

    Affinity and Kinetic Characterization of 84101-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84101-5-RR against Human Complement factor B were performed. The affinity constant is 2.27 nM.

Complement factor B Recombinant antibody

Complement factor B Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84101-5-RR

产品说明书

CloneNo. 241141A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CFB, CFAB, C3/C5 convertase, BFD, BF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse serum, rat serum, rat plasma, human plasma
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84101-5-RR targets Complement factor B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Complement factor B fusion protein Ag0226 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement factor B
别名 CFB, CFAB, C3/C5 convertase, BFD, BF
计算分子量 86 kDa
观测分子量 93-100 kDa
GenBank蛋白编号BC007990
基因名称 Complement factor B
Gene ID (NCBI) 629
RRIDAB_3671666
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00751
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease that is associated with C3b and forms the alternative pathway for C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

实验方案

Product Specific Protocols
WB protocol for Complement factor B antibody 84101-5-RRDownload protocol
IHC protocol for Complement factor B antibody 84101-5-RRDownload protocol
IF protocol for Complement factor B antibody 84101-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols