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验证数据展示

  • WB analysis using 85704-1-RR

    WB analysis using 85704-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 85704-1-RR

    WB analysis of human heart using 85704-1-RR

    human heart tissue were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85704-1-RR

    WB analysis using 85704-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 85704-1-RR

    IHC staining of human colon using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IHC staining of human colon using 85704-1-RR

    IHC staining of human colon using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IHC staining of mouse liver using 85704-1-RR

    IHC staining of mouse liver using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IHC staining of mouse liver using 85704-1-RR

    IHC staining of mouse liver using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included

COL6A1 Recombinant monoclonal antibody

COL6A1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85704-1-RR

产品说明书

CloneNo. 242936H3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman kidney tissue, PC-3 cells, human heart tissue, mouse kidney tissue, mouse heart tissue
Positive IHC detected inmouse liver tissue, human colon tissue, mouse colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85704-1-RR targets COL6A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11809

Product name: Recombinant human COL6A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 770-1028 aa of BC052575

Sequence: NVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type VI, alpha 1
别名 COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI
计算分子量 1028 aa, 109 kDa
观测分子量 140 kDa
GenBank蛋白编号BC052575
基因名称 COL6A1
Gene ID (NCBI) 1291
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12109
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
IHC protocol for COL6A1 antibody 85704-1-RRDownload protocol
WB protocol for COL6A1 antibody 85704-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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