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验证数据展示

  • WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

    WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 85704-1-RR (same clone as 85704-1-PBS)

    WB analysis of human heart using 85704-1-RR (same clone as 85704-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

  • WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

    WB analysis using 85704-1-RR (same clone as 85704-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

    IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

    IHC staining of mouse liver using 85704-1-RR (same clone as 85704-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85704-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL6A1 antibody included

COL6A1 Recombinant antibody, PBS Only

COL6A1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85704-1-PBS

产品说明书

CloneNo. 242936H3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.

产品信息

85704-1-PBS targets COL6A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 COL6A1 fusion protein Ag11809 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type VI, alpha 1
别名 COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI
计算分子量 1028 aa, 109 kDa
观测分子量 140 kDa
GenBank蛋白编号BC052575
基因名称 COL6A1
Gene ID (NCBI) 1291
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12109
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.