验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, mouse colon tissue, HeLa cells, pig colon tissue, rat colon tissue, Saos-2 cells, SiHa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy COL5A1 Ready-To-Use IHC Kit. COL5A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67604-1-Ig targets COL5A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type V, alpha 1 |
| 别名 | 2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V |
| 计算分子量 | 184 kDa |
| 观测分子量 | 220 kDa |
| GenBank蛋白编号 | NM_000093 |
| 基因名称 | COL5A1 |
| Gene ID (NCBI) | 1289 |
| RRID | AB_2882810 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20908 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
| IHC protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
| IF protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. | ||
Exp Ther Med COL3A1, COL5A1 and COL6A2 serve as potential molecular biomarkers for osteoarthritis based on weighted gene co‑expression network analysis bioinformatics analysis | ||
Neuropathology Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains. | ||
Comput Biol Med Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma | ||
J Adv Res Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4 | ||
Cell Signal Activated PARP1/FAK/COL5A1 signaling facilitates the tumorigenesis of cholesterol-resistant ovarian cancer cells through promoting EMT |
