验证数据展示
经过测试的应用
Positive WB detected in | HT-1080 cells, mouse colon tissue, HeLa cells, pig colon tissue, rat colon tissue, Saos-2 cells, SiHa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67604-1-Ig targets COL5A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat, pig |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | collagen, type V, alpha 1 |
别名 | 2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V |
计算分子量 | 184 kDa |
观测分子量 | 220 kDa |
GenBank蛋白编号 | NM_000093 |
基因名称 | COL5A1 |
Gene ID (NCBI) | 1289 |
RRID | AB_2882810 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P20908 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
IHC protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
IF protocol for COL5A1 antibody 67604-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. | ||
Exp Ther Med COL3A1, COL5A1 and COL6A2 serve as potential molecular biomarkers for osteoarthritis based on weighted gene co‑expression network analysis bioinformatics analysis | ||
Neuropathology Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains. | ||
Comput Biol Med Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma | ||
J Adv Res Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4 | ||
Cell Signal Activated PARP1/FAK/COL5A1 signaling facilitates the tumorigenesis of cholesterol-resistant ovarian cancer cells through promoting EMT |