COL5A1 Monoclonal antibody

COL5A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67604-1-Ig

产品说明书

CloneNo. 2C2C2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA and More (1)

2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-1080 cells, mouse colon tissue, HeLa cells, pig colon tissue, rat colon tissue, Saos-2 cells, SiHa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy COL5A1 Ready-To-Use IHC Kit. COL5A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67604-1-Ig targets COL5A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type V, alpha 1
别名 2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V
计算分子量 184 kDa
观测分子量 220 kDa
GenBank蛋白编号NM_000093
基因名称 COL5A1
Gene ID (NCBI) 1289
RRIDAB_2882810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20908
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COL5A1 antibody 67604-1-IgDownload protocol
IHC protocol for COL5A1 antibody 67604-1-IgDownload protocol
IF protocol for COL5A1 antibody 67604-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Theranostics

Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

Authors - Yinan Chen
humanIHC

Exp Ther Med

COL3A1, COL5A1 and COL6A2 serve as potential molecular biomarkers for osteoarthritis based on weighted gene co‑expression network analysis bioinformatics analysis

Authors - Yufeng Zhang
humanIHC

Neuropathology

Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains.

Authors - Keiji Wakamatsu
humanWB,IHC,IF

Comput Biol Med

Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma

Authors - Guokun Zhang

J Adv Res

Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4

Authors - Yidong Chen
human,mouseWB

Cell Signal

Activated PARP1/FAK/COL5A1 signaling facilitates the tumorigenesis of cholesterol-resistant ovarian cancer cells through promoting EMT

Authors - Zeyin He
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