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验证数据展示

  • WB analysis using 31615-1-AP

    WB analysis using 31615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31615-1-AP

    WB analysis using 31615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 31615-1-AP

    IHC staining of mouse liver using 31615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 31615-1-AP

    IHC staining of mouse liver using 31615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 31615-1-AP

    IF Staining of HepG2 using 31615-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Coagulation Factor II antibody (31615-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Coagulation Factor II Polyclonal antibody

Coagulation Factor II Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31615-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, PT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma, mouse heart tissue, rat plasma, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31615-1-AP targets Coagulation Factor II in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coagulation factor II
别名 F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, PT
计算分子量70 kDa
观测分子量 70-80 kDa
GenBank蛋白编号NM_010168.3
基因名称 F2
Gene ID (NCBI) 14061
RRIDAB_3670049
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19221
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis. This antibody specifically recognizes mouse Coagulation Factor II.

实验方案

Product Specific Protocols
WB protocol for Coagulation Factor II antibody 31615-1-APDownload protocol
IHC protocol for Coagulation Factor II antibody 31615-1-APDownload protocol
IF protocol for Coagulation Factor II antibody 31615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols