验证数据展示
经过测试的应用
| Positive WB detected in | Staurosporine treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85770-1-RR targets Cleaved Caspase 4/P20 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | caspase 4, apoptosis-related cysteine peptidase |
| 别名 | CASP4, Caspase4, CASP 4, CASP-4, Caspase 4 |
| 计算分子量 | 377 aa, 43 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC017839 |
| 基因名称 | Caspase 4 |
| Gene ID (NCBI) | 837 |
| RRID | AB_3744362 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49662 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase-4 participates in cleavage and activates gasdermin D (GSDMD), leading to cell membrane perforation, cell swelling and rupture, thereby inducing pyroptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cleaved Caspase 4/P20 antibody 85770-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
