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验证数据展示

  • WB analysis of Jurkat using RMX00003

    WB analysis of Jurkat using RMX00003

    Staurosporine treated and untreated Jurkat cells were subjected to SDS PAGE followed by western blot with RMX00003 (Cleaved Caspase 3/P17/P19) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using RMX00003

    IHC staining of mouse brain using RMX00003

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00003 (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cleaved Caspase 3 antibody included
  • IHC staining of mouse brain using RMX00003

    IHC staining of mouse brain using RMX00003

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00003 (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cleaved Caspase 3 antibody included
  • IF Staining of HeLa using RMX00003

    IF Staining of HeLa using RMX00003

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3/P17/P19 antibody (RMX00003) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Multi-rAb™ Cleaved Caspase 3/P17/P19 Multi-Recombinant antibody

Cleaved Caspase 3/P17/P19 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00003

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cleaved Caspase 3 Ready-To-Use IHC Kit. Cleaved Caspase 3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00003 targets Cleaved Caspase 3/P17/P19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 caspase 3, apoptosis-related cysteine peptidase
别名 CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3
计算分子量 32 kDa
观测分子量17 kDa, 19 kDa
GenBank蛋白编号NM_032991
基因名称 Caspase 3
Gene ID (NCBI) 836
偶联类型 Unconjugated
形式 Liquid
纯化方式N/A
UNIPROT IDP42574
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

实验方案

Product Specific Protocols
WB protocol for Cleaved Caspase 3/P17/P19 antibody RMX00003Download protocol
IHC protocol for Cleaved Caspase 3/P17/P19 antibody RMX00003Download protocol
IF protocol for Cleaved Caspase 3/P17/P19 antibody RMX00003Download protocol
Standard Protocols
Click here to view our Standard Protocols