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验证数据展示

  • WB analysis using 25128-1-AP (same clone as 25128-1-PBS)

    WB analysis using 25128-1-AP (same clone as 25128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25128-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 25128-1-AP (same clone as 25128-1-PBS)

    IHC staining of mouse brain using 25128-1-AP (same clone as 25128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25128-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cleaved Caspase 3 antibody included
  • IF Staining of HeLa using 25128-1-AP (same clone as 25128-1-PBS)

    IF Staining of HeLa using 25128-1-AP (same clone as 25128-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (25128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 25128-1-PBS in a different storage buffer formulation.

Cleaved Caspase 3 Polyclonal antibody, PBS Only

Cleaved Caspase 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25128-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CASP3, Caspase3, CASP 3, CASP-3, Caspase 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Cleaved Caspase 3 Ready-To-Use IHC Kit. Cleaved Caspase 3 primary antibody included.

产品信息

25128-1-PBS targets Cleaved Caspase 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 3, apoptosis-related cysteine peptidase
别名 CASP3, Caspase3, CASP 3, CASP-3, Caspase 3
计算分子量 32 kDa
观测分子量17-25 kDa
GenBank蛋白编号NM_032991
基因名称 Caspase 3
Gene ID (NCBI) 836
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42574
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).