验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29999-1-AP targets Claudin 5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag29731 Product name: Recombinant human CLDN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-50 aa of BC032363 Sequence: MGSAALEILGLVLCLVGWGGLILACGLPMWQVTAFLDHNIVTAQTTWKGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | claudin 5 |
| 别名 | CLDN5, AWAL, BEC1, Claudin5, Claudin-5 |
| 计算分子量 | 218 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC032363 |
| 基因名称 | CLDN5 |
| Gene ID (NCBI) | 7122 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00501 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. Claudin-5 is a tight junction protein primarily expressed in endothelial cells, forming the blood-brain barrier. It regulates paracellular permeability and is crucial for vascular integrity. Altered expression is linked to blood-brain barrier disruption in neurological diseases and cancer metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 5 antibody 29999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
