验证数据展示

WB analysis using 29767-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 29767-1-AP
hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 29767-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 29767-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CLDN5 antibody included
IHC staining of human colon using 29767-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CLDN5 antibody included
IHC staining of mouse lung using 29767-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CLDN5 antibody included
IHC staining of mouse lung using 29767-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CLDN5 antibody included
IF Staining of mouse lung using 29767-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Claudin 5 antibody (29767-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 29767-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Claudin 5 antibody (29767-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Claudin 5 Polyclonal antibody

Claudin 5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 29767-1-AP

产品说明书

引用文献(99)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF-P, ELISA and More (1)

别名

CLDN5, AWAL, BEC1, Claudin5, Claudin-5

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (99)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	unboiled mouse lung tissue, hTERT-RPE1 cells, rat lung tissue, unboiled rat lung tissue
Positive IHC detected in	human colon tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse lung tissue, mouse brain tissue

Planning an IHC experiment? We recommend our IHCeasy CLDN5 Ready-To-Use IHC Kit. CLDN5 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 74 publications below
IHC	See 4 publications below
IF	See 17 publications below

产品信息

29767-1-AP targets Claudin 5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF-P, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, bovine, sheep
免疫原	CatNo: Ag29044 Product name: Recombinant human CLDN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 144-218 aa of BC032363 Sequence: VREFYDPSVPVSQKYELGAALYIGWAATALLMVGGCLLCCGAWVCTGRPDLSFPVKYSAPRRPTATGDYDKKNYV 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	claudin 5
别名	CLDN5, AWAL, BEC1, Claudin5, Claudin-5
计算分子量	218 aa, 23 kDa
观测分子量	23 kDa
GenBank蛋白编号	BC032363
基因名称	CLDN5
Gene ID (NCBI)	7122
RRID	AB_2935477
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	O00501
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. Claudin-5 is a tight junction protein primarily expressed in endothelial cells, forming the blood-brain barrier. It regulates paracellular permeability and is crucial for vascular integrity. Altered expression is linked to blood-brain barrier disruption in neurological diseases and cancer metastasis.

实验方案

Product Specific Protocols
IF protocol for Claudin 5 antibody 29767-1-AP	Download protocol
IHC protocol for Claudin 5 antibody 29767-1-AP	Download protocol
WB protocol for Claudin 5 antibody 29767-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
		Metabolism Setmelanotide alleviates diabetes-associated cognitive impairment by interrupting the binding of ICAM-1 and JIP1 in endothelial cells of the blood-brain barrier. Authors - Shi-Di Wu View Article
mouse	WB	Redox Biol SLC22A3 deficiency leads to cognitive impairment through the cardio-neuroinflammatory axis mediated HA/H1R/NLRP3 pathway in heart failure mice. Authors - Zhitian Wang View Article
mouse,human	WB	Cardiovasc Diabetol Pgk1 activation restores endothelial metabolic homeostasis to alleviate vascular aging and atherosclerosis. Authors - Jinhong Lu View Article
rat	WB	J Nanobiotechnology tLyP-1 peptide-modified MnO₂ co-delivering si-SLC16A1 and temozolomide synergistically suppresses glioblastoma via hypoxia modulation and metabolic stress. Authors - Haiting Zhao View Article
		J Nanobiotechnology Targeting microglial circMBNL1 unlocks a novel nanomedicine therapeutic strategy for major depressive disorder. Authors - Xiaoyu Yu View Article
		Adv Sci (Weinh) Bifidobacterium Pseudolongum-Derived Inosine Mitigates Polystyrene Nanoplastics-Induced Hepatic Injury by Inhibiting the Polarization of M1 Macrophages. Authors - Kaikai Zhang View Article

验证数据展示

WB analysis using 29767-1-AP

WB analysis using 29767-1-AP

WB analysis using 29767-1-AP

IHC staining of human colon using 29767-1-AP

IHC staining of human colon using 29767-1-AP

IHC staining of mouse lung using 29767-1-AP

IHC staining of mouse lung using 29767-1-AP

IF Staining of mouse lung using 29767-1-AP

IF Staining of mouse brain using 29767-1-AP

Claudin 5 Polyclonal antibody

Claudin 5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Setmelanotide alleviates diabetes-associated cognitive impairment by interrupting the binding of ICAM-1 and JIP1 in endothelial cells of the blood-brain barrier.

SLC22A3 deficiency leads to cognitive impairment through the cardio-neuroinflammatory axis mediated HA/H1R/NLRP3 pathway in heart failure mice.

Pgk1 activation restores endothelial metabolic homeostasis to alleviate vascular aging and atherosclerosis.

tLyP-1 peptide-modified MnO₂ co-delivering si-SLC16A1 and temozolomide synergistically suppresses glioblastoma via hypoxia modulation and metabolic stress.

Targeting microglial circMBNL1 unlocks a novel nanomedicine therapeutic strategy for major depressive disorder.

Bifidobacterium Pseudolongum-Derived Inosine Mitigates Polystyrene Nanoplastics-Induced Hepatic Injury by Inhibiting the Polarization of M1 Macrophages.

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