验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, mouse colon tissue, Calu-3 cells, MCF-7 cells, rat colon tissue |
| Positive IP detected in | HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
86697-1-RR targets Claudin 3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | claudin 3 |
| 别名 | CLDN3, Claudin3, Claudin-3, CPE R 2, CPE R2 |
| 计算分子量 | 220 aa, 23 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC016056 |
| 基因名称 | Claudin 3 |
| Gene ID (NCBI) | 1365 |
| RRID | AB_3745061 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O15551 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudin-3 (CLDN3) is one of 27 known members of the claudin family. Together with occludin and other junctional adhesion molecules, the claudins form the tight-junctions (TJs) that regulate intercellular permeability. Altered CLDN3 expression has been linked to tumor progression in multiple tumor types.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for Claudin 3 antibody 86697-1-RR | Download protocol |
| WB protocol for Claudin 3 antibody 86697-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
