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验证数据展示

  • WB analysis using 28663-1-AP

    WB analysis using 28663-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 28663-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-12 using 28663-1-AP

    FC experiment of PC-12 using 28663-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (28663-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Chromogranin B Polyclonal antibody

Chromogranin B Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 28663-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

CgB, CHGB, SCG1, Chromogranin-B, PE-11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-12 cells
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy CHGB Ready-To-Use IHC Kit. CHGB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28663-1-AP targets Chromogranin B in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag29920

Product name: Recombinant human CHGB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-328 aa of BC000375

Sequence: MPVDNRNHNEGMVTRCIIEVLSNALSKSSAPPITPECRQVLKTSRKDVKDKETTENENTKFEVRLLRDPADASEAHESSSRGEAGAPGEEDIQGPTKADTEKWAEGGGHSRERADEPQWSLYPSDSQVSEEVKTRHSEKSQREDEEEEEGENYQKGERGEDSSEEKHLEEPGETQNAFLNERKQASAIKKEELVARSETHAAGHSQEKTHSREKSSQESGEEAGSQENHPQESKGQPRSQEESEEGEEDATSEVDKRRTRPRHHHGRSRPDRSSQGGSLPSEEKGHPQEESEESNVSMASLGEKRDHH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromogranin B (secretogranin 1)
别名 CgB, CHGB, SCG1, Chromogranin-B, PE-11
计算分子量 78 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC000375
基因名称 Chromogranin B
Gene ID (NCBI) 1114
RRIDAB_2881188
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05060
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for Chromogranin B antibody 28663-1-APDownload protocol
WB protocol for Chromogranin B antibody 28663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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