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验证数据展示

  • WB analysis using 85605-2-RR

    WB analysis using 85605-2-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85605-2-RR

    WB analysis using 85605-2-RR

    TT cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85605-2-RR

    WB analysis using 85605-2-RR

    PC-12 cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85605-2-RR

    WB analysis using 85605-2-RR

    Pig adrenal gland tissue was subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 85605-2-RR

    IHC staining of mouse pancreas using 85605-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of human appendix using 85605-2-RR

    IHC staining of human appendix using 85605-2-RR

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of mouse colon using 85605-2-RR

    IHC staining of mouse colon using 85605-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of human colon using 85605-2-RR

    IHC staining of human colon using 85605-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of human pancreas using 85605-2-RR

    IHC staining of human pancreas using 85605-2-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IHC staining of human pancreas using 85605-2-RR

    IHC staining of human pancreas using 85605-2-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CgA/CHGA antibody included
  • IF Staining of mouse pancreas using 85605-2-RR

    IF Staining of mouse pancreas using 85605-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 85605-2-RR

    IF Staining of mouse small intestine using 85605-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Chromogranin A Recombinant antibody

Chromogranin A Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85605-2-RR

产品说明书

CloneNo. 243048H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

CGA, CHGA, AL-11, Chromogranin-A, EA-92

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, TT cells, PC-12 cells, pig adrenal gland tissue
Positive IHC detected inhuman pancreas tissue, human appendix tissue, human colon tissue, mouse colon tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, mouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy CgA/CHGA Ready-To-Use IHC Kit. CgA/CHGA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85605-2-RR targets Chromogranin A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Chromogranin A fusion protein Ag19780 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromogranin A (parathyroid secretory protein 1)
别名 CGA, CHGA, AL-11, Chromogranin-A, EA-92
计算分子量 51 kDa
观测分子量 70-85 kDa
GenBank蛋白编号BC006459
基因名称 Chromogranin A
Gene ID (NCBI) 1113
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

实验方案

Product Specific Protocols
WB protocol for Chromogranin A antibody 85605-2-RRDownload protocol
IHC protocol for Chromogranin A antibody 85605-2-RRDownload protocol
IF protocol for Chromogranin A antibody 85605-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols