Validation Data Gallery
![Live A431 cells were incubated with anti-human EGFR (Cetuximab biosimilar) followed by Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, orange). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 40x objective and post-processed.](/products/pictures/Result-shuGCL555-2.jpg "Live A431 cells were incubated with anti-human EGFR (Cetuximab biosimilar) followed by Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, orange). Cells were fixed and nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 40x objective and post-processed.")
![Immunofluorescence analysis of transgenic CHO-PD1 cells. PFA-fixed cells were stained with anti-human PD1 (Pembrolizumab biosimilar), Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, red), and DAPI (grey).](/products/pictures/Result-shuGCL555-2_20231123142425221.jpg "Immunofluorescence analysis of transgenic CHO-PD1 cells. PFA-fixed cells were stained with anti-human PD1 (Pembrolizumab biosimilar), Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (shuGCL555-2, red), and DAPI (grey).")
Product Information
Nano-Secondary® anti-human IgG, recombinant VHH, is a human IgG-specific (IgG1, IgG2, IgG3, IgG4) antibody. This secondary antibody product consists of a Nanobody that binds to the Fc-domain of human IgG with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-human IgG, Fc-specific recombinant VHH reagent uses a novel class of human IgG-specific antibodies. This secondary antibody product consists of a Nanobody that binds to human IgGs (IgG1, IgG2, IgG3, IgG4) with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species apart from slight reactivity to macaque serum, details see below. |
| Applications | IF, FC |
| Host | Alpaca |
| Species Reactivity | Reactivity: human IgG1, IgG2, IgG3, IgG4, Fc-fragment of human IgG, including wt, PGLALA and PALALA mutations. Slight reactivity to macaque serum. No cross-reactivity: human IgA/IgM/IgE, serum proteins from goat, guinea pig, mouse rabbit, rat, sheep. |
| Target/Specificity | Fc-fragment of human IgG (IgG1, IgG2, IgG3, IgG4) |
| Physical State | Liquid |
| Conjugate | CoraLite® Plus 555 |
| Suggested Dilution | Immunofluorescence 1:500 - 1:1000 Flow cytometry: 1µL per 1x106 cells |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Secondary Nanobody |
| Class | Recombinant |
| Format | Alpaca single domain antibody, monovalent |
| Cross-reactivity | No cross-reactivity to human IgA/IgM/IgE, and goat, guinea pig, mouse, rabbit, rat, and sheep serum. Shows slight cross-reactivity to macaque serum. |
| Immunogen | Purified human IgG |
| Clonality | Monoclonal Nanobody |
| Clones | CTK0117 (VHH1118) |
| RRID | AB_3075303 |
| Affinity (KD) | < 10 pM |
| Excitation / Emission | Excitation max: 554 nm, Emission max: 570 nm |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C. |
| Size | 10 μL; 100 μL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| shuGCL555-2_SDS_Nano-Secondary® alpaca anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] (EN) |
| Datasheet |
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| Nano-Secondary® anti-human IgG, recombinant VHH, CoraLite® Plus 555 [CTK0117] Datasheet |
| Application note |
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| One-step Incubation with Nano-Secondaries (PDF) |
| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |