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验证数据展示

  • WB analysis of 3T3-L1 using 27293-1-AP (same clone as 27293-1-PBS)

    WB analysis of 3T3-L1 using 27293-1-AP (same clone as 27293-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27293-1-AP (CHRD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

    IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

    IHC staining of human liver cancer using 27293-1-AP (same clone as 27293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27293-1-AP (Chordin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27293-1-PBS in a different storage buffer formulation.

Chordin Polyclonal antibody, PBS Only

Chordin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27293-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27293-1-PBS targets Chordin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26001

Product name: Recombinant human CHRD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-350 aa of BC112345

Sequence: AGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chordin
别名
计算分子量 955 aa, 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC112345
基因名称 Chordin
Gene ID (NCBI) 8646
RRIDAB_2880832
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2X0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration.

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