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验证数据展示

  • WB analysis using 84905-1-RR

    WB analysis using 84905-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84905-1-RR (CHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84905-1-RR

    IF Staining of HeLa using 84905-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHK1 antibody (84905-1-RR, Clone: 240618E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of K-562 using 84905-1-RR

    FC experiment of K-562 using 84905-1-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Chk1 Recombinant antibody (84905-1-RR, Clone:240618E11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Chk1 Recombinant antibody

Chk1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84905-1-RR

产品说明书

CloneNo. 240618E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells, A549 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84905-1-RR targets Chk1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Chk1 fusion protein Ag22993 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名 CHEK1, CHK1 checkpoint homolog, Chk 1, CHEK 1, Checkpoint kinase-1
计算分子量 54 kDa
观测分子量 55 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO14757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.

实验方案

Product Specific Protocols
WB protocol for Chk1 antibody 84905-1-RRDownload protocol
IF protocol for Chk1 antibody 84905-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols