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验证数据展示

  • WB analysis of PC-12 using 86447-1-RR (same clone as 86447-1-PBS)

    WB analysis of PC-12 using 86447-1-RR (same clone as 86447-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 86447-1-RR (Chga antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86447-1-PBS in a different storage buffer formulation.

  • Sample test of MP02444-1

    Sample test of MP02444-1

    The mean Chromogranin a concentration was determined to be 77.6 ng/mL in mouse serum.

  • IHC staining of mouse pancreas using 86447-1-RR (same clone as 86447-1-PBS)

    IHC staining of mouse pancreas using 86447-1-RR (same clone as 86447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 86447-1-RR (Chromogranin A antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86447-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 86447-1-RR (same clone as 86447-1-PBS)

    IF Staining of mouse pancreas using 86447-1-RR (same clone as 86447-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Chromogranin A antibody (86447-1-RR, Clone: 250318B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86447-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02444-1

    Cytometric bead array standard curve of MP02444-1

    Cytometric bead array standard curve of MP02444-1, MOUSE Chromogranin a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86447-1-PBS. Detection antibody: 86447-2-PBS. Standard: Eg2760. Range: 0.625-80 ng/mL.

Mouse Chromogranin A Recombinant monoclonal antibody, PBS Only (Capture)

Chromogranin A Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 86447-1-PBS

产品说明书

CloneNo. 250318B12

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

别名

Beta-granin, Catestatin, CgA, Chga, Chromogranin-A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86447-1-PBS targets Chromogranin A as part of a matched antibody pair:

MP02444-1: 86447-1-PBS capture and 86447-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2760

Product name: recombinant mouse Chga protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 19-463 aa of NM_007693.2

Sequence: LPVNSPMTKGDTKVMKCVLEVISDSLSKPSPMPVSPECLETLQGDERILSILRHQNLLKELQDLALQGAKERAQQPLKQQQPPKQQQQQQQQQQQEQQHSSFEDELSEVFENQSPDAKHRDAAAEVPSRDTMEKRKDSDKGQQDGFEATTEGPRPQAFPEPNQESPMMGDSESPGEDTATNTQSPTSLPSQEHVDPQATGDSERGLSAQQQARKAKQEEKEEEEEEEAVAREKAGPEEVPTAASSSHFHAGYKAIQKDDGQSDSQAVDGDGKTEASEALPSEGKGELEHSQQEEDGEEAMVGTPQGLFPQGGKGRELEHKQEEEEEEEERLSREWEDKRWSRMDQLAKELTAEKRLEGEDDPDRSMKLSFRTRAYGFRDPGPQLRRGWRPSSREDSVEARSDFEEKKEEEGSANRRAEDQELESLSAIEAELEKVAHQLQALRRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromogranin A
别名 Beta-granin, Catestatin, CgA, Chga, Chromogranin-A
计算分子量52 kDa
观测分子量 70-85 kDa
GenBank蛋白编号NM_007693.2
基因名称 Chga
Gene ID (NCBI) 12652
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26339
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

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