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验证数据展示

  • WB analysis using 83550-4-RR (same clone as 83550-4-PBS)

    WB analysis using 83550-4-RR (same clone as 83550-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83550-4-RR (Chemerin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83550-4-PBS in a different storage buffer formulation.

  • FC experiment of C57BL/6 mouse liver cells using 83550-4-RR (same clone as 83550-4-PBS)

    FC experiment of C57BL/6 mouse liver cells using 83550-4-RR (same clone as 83550-4-PBS)

    1x10^6 C57BL/6 mouse liver cells were intracellularly stained with 0.25 ug Chemerin Recombinant antibody (83550-4-RR, Clone:240355A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83550-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83550-4-RR

    Affinity and Kinetic Characterization of 83550-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83550-4-RR against Mouse Chemerin were performed. The affinity constant is 3.76 nM.

Mouse Chemerin Recombinant monoclonal antibody, PBS Only

Chemerin Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83550-4-PBS

产品说明书

CloneNo. 240355A12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, FC (Intra), ELISA

别名

Rarres2, 240355A12, AI303516, Retinoic acid receptor responder protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83550-4-PBS targets Chemerin in WB, FC (Intra), ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg0875

Product name: Recombinant mouse Chemerin protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-156 aa of NM_001347168.1

Sequence: EPELSETQRRSLQVALEEFHKHPPVQLAFQEIGVDRAEEVLFSAGTFVRLEFKLQQTNCPKKDWKKPECTIKPNGRRRKCLACIKMDPKGKILGRIVHCPILKQGPQDPQELQCIKIAQAGEDPHGYFLPGQFAFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoic acid receptor responder (tazarotene induced) 2
别名 Rarres2, 240355A12, AI303516, Retinoic acid receptor responder protein 2
计算分子量18 kDa
GenBank蛋白编号NM_001347168.1
基因名称 Chemerin
Gene ID (NCBI) 71660
RRIDAB_3671168
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9DD06
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. RARRES2 have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. The MW of Chemerin is 14-19 kDa.

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