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验证数据展示

  • IF Staining of hTERT-RPE1 using 68433-1-Ig (same clone as 68433-1-PBS)

    IF Staining of hTERT-RPE1 using 68433-1-Ig (same clone as 68433-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68433-1-PBS in a different storage buffer formulation.

  • IF Staining of ARPE-19 using 68433-1-Ig (same clone as 68433-1-PBS)

    IF Staining of ARPE-19 using 68433-1-Ig (same clone as 68433-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68433-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 68433-1-Ig (same clone as 68433-1-PBS)

    IF Staining of hTERT-RPE1 using 68433-1-Ig (same clone as 68433-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68433-1-PBS in a different storage buffer formulation.

Centrin 1 Monoclonal antibody, PBS Only

Centrin 1 Monoclonal Antibody for IF/ICC, Indirect ELISA
Cat No. 68433-1-PBS

产品说明书

CloneNo. 1G8D10

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF/ICC, Indirect ELISA

别名

Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68433-1-PBS targets Centrin 1 in IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag3529

Product name: Recombinant human CETN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-172 aa of BC029515

Sequence: MASGFKKPSAASTGQKRKVAPKPELTEDQKQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFNDFLAVMTQKMSEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEELQEMIDEADRDGDGEVNEEEFLRIMKKTSLY

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 centrin, EF-hand protein, 1
别名 Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1
计算分子量 172 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC029515
基因名称 Centrin 1
Gene ID (NCBI) 1068
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12798
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

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