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验证数据展示

  • WB analysis using 84044-1-RR

    WB analysis using 84044-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84044-1-RR (Caveolin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 84044-1-RR

    IHC staining of human lung using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human spleen using 84044-1-RR

    IHC staining of human spleen using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human stomach cancer using 84044-1-RR

    IHC staining of human stomach cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human breast cancer using 84044-1-RR

    IHC staining of human breast cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 84044-1-RR

    IHC staining of human ovary cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 84044-1-RR

    IHC staining of human tonsillitis using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 84044-1-RR

    IHC staining of human tonsillitis using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human liver using 84044-1-RR

    IHC staining of human liver using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 84044-1-RR

    IHC staining of human appendicitis using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 84044-1-RR

    IHC staining of human appendicitis using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human lung squamous cell carcinoma using 84044-1-RR

    IHC staining of human lung squamous cell carcinoma using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human lung using 84044-1-RR

    IHC staining of human lung using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human tonsillitis using 84044-1-RR

    IHC staining of human tonsillitis using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human tonsillitis using 84044-1-RR

    IHC staining of human tonsillitis using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human ovary cancer using 84044-1-RR

    IHC staining of human ovary cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human breast cancer using 84044-1-RR

    IHC staining of human breast cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human placenta using 84044-1-RR

    IHC staining of human placenta using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human stomach cancer using 84044-1-RR

    IHC staining of human stomach cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human colon using 84044-1-RR

    IHC staining of human colon using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human colon cancer using 84044-1-RR

    IHC staining of human colon cancer using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human spleen using 84044-1-RR

    IHC staining of human spleen using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • IHC staining of human liver using 84044-1-RR

    IHC staining of human liver using 84044-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:10000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-1 antibody included
  • Affinity and Kinetic Characterization of 84044-1-RR

    Affinity and Kinetic Characterization of 84044-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84044-1-RR against Human Caveolin-1 were performed. The affinity constant is 1.53 nM.

Caveolin-1 Recombinant monoclonal antibody

Caveolin-1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84044-1-RR

产品说明书

CloneNo. 240985E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CAV1, Caveolin 1, 240985E4, BSCL3, CAV

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A549 cells, MDA-MB-231 cells
Positive IHC detected inhuman colon cancer tissue, human appendicitis tissue, human placenta tissue, human liver tissue, human ovary cancer tissue, human lung squamous cell carcinoma tissue, human lung tissue, human tonsillitis tissue, human breast cancer tissue, human colon tissue, human spleen tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Caveolin-1 Ready-To-Use IHC Kit. Caveolin-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84044-1-RR targets Caveolin-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8049

Product name: Recombinant human Caveolin-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 11-179 aa of BC006432

Sequence: GHLYTVPIREQGNIYKPNNKAMADELSEKQVYDAHTKEIDLVNRDPKHLNDDVVKIDFEDVIAEPEGTHSFDGIWKASFTTFTVTKYWFYRLLSALFGIPMALIWGIYFAILSFLHIWAVVPCIKSFLIEIQCISRVYSIYVHTVCDPLFEAVGKIFSNVRINLQKEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 caveolin 1, caveolae protein, 22kDa
别名 CAV1, Caveolin 1, 240985E4, BSCL3, CAV
计算分子量 22 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC006432
基因名称 CAV1
Gene ID (NCBI) 857
RRIDAB_3671611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ03135
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

实验方案

Product Specific Protocols
IHC protocol for Caveolin-1 antibody 84044-1-RRDownload protocol
WB protocol for Caveolin-1 antibody 84044-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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