×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 11239-1-AP (same clone as 11239-1-PBS)

    WB analysis using 11239-1-AP (same clone as 11239-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 11239-1-AP (Cathepsin K antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 11239-1-AP (same clone as 11239-1-PBS)

    IF Staining of mouse brain using 11239-1-AP (same clone as 11239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • IP experiment of ROS1728 using 11239-1-AP (same clone as 11239-1-PBS)

    IP experiment of ROS1728 using 11239-1-AP (same clone as 11239-1-PBS)

    IP result of anti-Cathepsin K (IP:11239-1-AP, 3ug; Detection:11239-1-AP 1:500) with ROS1728 cells lysate 3200ug. This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 11239-1-AP (same clone as 11239-1-PBS)

    IHC staining of human breast cancer using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 11239-1-AP (same clone as 11239-1-PBS)

    IHC staining of human breast cancer using 11239-1-AP (same clone as 11239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 11239-1-AP (same clone as 11239-1-PBS)

    IF Staining of mouse brain using 11239-1-AP (same clone as 11239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • IF Staining of ROS1728 using 11239-1-AP (same clone as 11239-1-PBS)

    IF Staining of ROS1728 using 11239-1-AP (same clone as 11239-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using Cathepsin K antibody (11239-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11239-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cathepsin K Polyclonal antibody, PBS Only

Cathepsin K Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 11239-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

CTSK, Cathepsin X, catK, CTSO, CTSO2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

11239-1-PBS targets Cathepsin K in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1761

Product name: Recombinant human CTSK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-329 aa of BC016058

Sequence: MWGLKVLLLPVVSFALYPEEILDTHWELWKKTHRKQYNNKVDEISRRLIWEKNLKYISIHNLEASLGVHTYELAMNHLGDMTSEEVVQKMTGLKVPLSHSRSNDTLYIPEWEGRAPDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEGQLKKKTGKLLNLSPQNLVDCVSENDGCGGGYMTNAFQYVQKNRGIDSEDAYPYVGQEESCMYNPTGKAAKCRGYREIPEGNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDESCNSDNLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFPKM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin K
别名 CTSK, Cathepsin X, catK, CTSO, CTSO2
计算分子量 39 kDa
观测分子量27 kDa, 38~46 kDa
GenBank蛋白编号BC016058
基因名称 Cathepsin K
Gene ID (NCBI) 1513
RRIDAB_2245581
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43235
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSK(cathepsin K), also named CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts (PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot (PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

||
New chat

Able™

正在加载，请稍候...