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验证数据展示

  • WB analysis using 31516-1-AP

    WB analysis using 31516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31516-1-AP (Cathepsin D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31516-1-AP

    IHC staining of human ovary cancer using 31516-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IHC staining of human ovary cancer using 31516-1-AP

    IHC staining of human ovary cancer using 31516-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IHC staining of human liver using 31516-1-AP

    IHC staining of human liver using 31516-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IHC staining of mouse liver using 31516-1-AP

    IHC staining of mouse liver using 31516-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IF Staining of A431 using 31516-1-AP

    IF Staining of A431 using 31516-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (31516-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IF Staining of MCF-7 using 31516-1-AP

    IF Staining of MCF-7 using 31516-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (31516-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31516-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, HuH-7 cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Cathepsin D Ready-To-Use IHC Kit. Cathepsin D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31516-1-AP targets Cathepsin D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1053

Product name: Recombinant Human Cathepsin D protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-412 aa of NM_001909.5

Sequence: LVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin D
别名 CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD
计算分子量45kDa
观测分子量32 kDa, 48 kDa, 52 kDa
GenBank蛋白编号NM_001909.5
基因名称 Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_3670015
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP07339
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTSD (cathepsin D) is a lysosomal aspartic protease in the pepsin superfamily ubiquitous to all cell types but particularly abundant in the central nervous system (CNS). The signal peptide of the pre-proCTSD (52 kDa) is cleaved in the endoplasmic reticulum (ER), forming the pro-CTSD (48 kDa), finally processed to form the mature CTSD (32 kDa) in the lysosome (PMID: 31282275,15126630). The major role of the m-CTSD is the digestion of internalized waste cell proteins and peptides in the lysosome, which maintains cell health (PMID: 32324083). CTSD is a well-established aspartyl protease that functions intracellularly in lysosomes and extracellularly in several forms of cancer.

实验方案

Product Specific Protocols
IF protocol for Cathepsin D antibody 31516-1-APDownload protocol
IHC protocol for Cathepsin D antibody 31516-1-APDownload protocol
WB protocol for Cathepsin D antibody 31516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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