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验证数据展示

  • WB analysis using 83998-4-RR (same clone as 83998-4-PBS)

    WB analysis using 83998-4-RR (same clone as 83998-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83998-4-RR (Cathepsin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83998-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Urine of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 1.64 ng/mL with a range of 0.76-2.39 ng/mL.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Saliva of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 186.38 ng/mL with a range of 146.98-271.31 ng/mL.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Human milk of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 150.72 ng/mL with a range of 95.82-192.06 ng/mL.

  • Sandwich ELISA sample test of MP03489-1

    Sandwich ELISA sample test of MP03489-1

    Plasma of six individual healthy human donors was measured. The Cathepsin B concentration of detected samples was determined to be 65.00 ng/mL with a range of 32.20-170.78 ng/mL.

  • Sandwich ELISA standard curve of MP03489-1

    Sandwich ELISA standard curve of MP03489-1

    Sandwich ELISA standard curve of MP03489-1, Human Cathepsin B Recombinant Matched Antibody Pair - PBS only. 83998-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1020. 83998-5-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • IP experiment of HepG2 using 83998-4-RR (same clone as 83998-4-PBS)

    IP experiment of HepG2 using 83998-4-RR (same clone as 83998-4-PBS)

    IP result of anti-Cathepsin B (IP:83998-4-RR, 4ug; Detection:83998-4-RR 1:600) with HepG2 cells lysate 1280 ug. This data was developed using the same antibody clone with 83998-4-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03489-1

    Cytometric bead array sample test of MP03489-1

    The mean Cathepsin B concentration was determined to be 41.1 ng/mL in human plasma.

  • Cytometric bead array standard curve of MP03489-1

    Cytometric bead array standard curve of MP03489-1

    Cytometric bead array standard curve of MP03489-1, Cathepsin B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83998-4-PBS. Detection antibody: 83998-5-PBS. Standard: Eg1020. Range: 0.078-10 ng/mL.

Cathepsin B Recombinant monoclonal antibody, PBS Only (Capture)

Cathepsin B Uni-rAb® Recombinant Antibody for WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 83998-4-PBS

产品说明书

CloneNo. 260854C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Cathepsin B Ready-To-Use IHC Kit. Cathepsin B primary antibody included.

产品信息

83998-4-PBS targets Cathepsin B as part of a matched antibody pair:

MP03489-1: 83998-4-PBS capture and 83998-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1020

Product name: Recombinant human Cathepsin B protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 18-339 aa of NM_001908.5

Sequence: RSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cathepsin B
别名 CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain
计算分子量38kDa
观测分子量31 kDa, 43 kDa
GenBank蛋白编号NM_001908.5
基因名称 Cathepsin B
Gene ID (NCBI) 1508
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07858
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

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