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验证数据展示

  • WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

    WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

    IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSA antibody included
  • IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

    IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSA antibody included
  • IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

    IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSA antibody included
  • IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

    IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSA antibody included
  • WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

    WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

    IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSA antibody included
  • IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

    IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTSA antibody included

Cathepsin A Polyclonal antibody, PBS Only

Cathepsin A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15020-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CTSA Ready-To-Use IHC Kit. CTSA primary antibody included.

产品信息

15020-1-PBS targets Cathepsin A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7073

Product name: Recombinant human CTSA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 133-479 aa of BC000597

Sequence: FSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin A
别名
计算分子量 54 kDa
观测分子量 54-60, 32, 20 kDa
GenBank蛋白编号BC000597
基因名称 Cathepsin A
Gene ID (NCBI) 5476
RRIDAB_10696309
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10619
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).

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