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验证数据展示

  • WB analysis of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

    WB analysis of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 13423-1-AP (same clone as 13423-1-PBS)

    IP experiment of HeLa using 13423-1-AP (same clone as 13423-1-PBS)

    IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

    IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP8 antibody included
  • IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

    IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASP8 antibody included
  • IF Staining of HepG2 using 13423-1-AP (same clone as 13423-1-PBS)

    IF Staining of HepG2 using 13423-1-AP (same clone as 13423-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/P43/P18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Caspase 8/P43/P18 Polyclonal antibody, PBS Only

Caspase 8/P43/P18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13423-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CASP8 Ready-To-Use IHC Kit. CASP8 primary antibody included.

产品信息

13423-1-PBS targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 Caspase 8/P43/P18 fusion protein Ag4214 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 8, apoptosis-related cysteine peptidase
别名 CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4
计算分子量 538 aa, 62 kDa
观测分子量 53-57 kDa, 32-45 kDa
GenBank蛋白编号BC028223
基因名称 Caspase 8
Gene ID (NCBI) 841
RRIDAB_2068463
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14790
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.