验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, LNCaP cells, PC-3 cells, MCF-7 cells, mouse liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27155-1-AP targets Caspase 7 in WB, IHC, IF/ICC, IF-P, IP, ELISA, Immunofluorescence, Western Blot applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, Immunofluorescence, Western Blot |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, monkey, chicken, bovine, sheep |
| 免疫原 |
CatNo: Ag25904 Product name: Recombinant human Caspase 7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC015799 Sequence: MADEQGCIEEQGVEDSANEDSVDAKPDRSSFVPSLFSKKKKNVTMRSIKTTRDRVPTYQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase 7, apoptosis-related cysteine peptidase |
| 别名 | CASP7, Apoptotic protease Mch 3, Apoptotic protease Mch-3, CASP 7, CASP-7 |
| 计算分子量 | 303 aa, 34 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC015799 |
| 基因名称 | Caspase 7 |
| Gene ID (NCBI) | 840 |
| RRID | AB_2880779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55210 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 7 antibody 27155-1-AP | Download protocol |
| IP protocol for Caspase 7 antibody 27155-1-AP | Download protocol |
| WB protocol for Caspase 7 antibody 27155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Selective Organ-Targeting Hafnium Oxide Nanoparticles with Multienzyme-Mimetic Activities Attenuate Radiation-Induced Tissue Damage | ||
Cell Stem Cell Psychological stress drives aging-like hematopoietic stem cell dysfunction through a brain-gut-bone marrow axis. | ||
Autophagy E-cigarette aerosols induce the hydrolysis of lysosomal glycerophospholipids through PLA2G4A activation initiated by nicotine binding to CHRNA3/α3 nAchr in airway epithelial cells. | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
J Adv Res PERK drives aflatoxin B1-induced chicken cellular senescence by promoting NLRP3-mediated PANoptosis. | ||
Gut Microbes Dietary cholesterol impairs cognition via gut microbiota-derived deoxycholic acid in obese mice |
