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验证数据展示

  • IF Staining of HUVEC using 22915-1-AP (same clone as 22915-1-PBS)

    IF Staining of HUVEC using 22915-1-AP (same clone as 22915-1-PBS)

    IF result of 22915-1-AP from publication with PMID: 31611559. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)

    IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)

    IHC staining of human spleen using 22915-1-AP (same clone as 22915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP (Caspase 1/P20 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

  • WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

    WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

  • WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

    WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

  • WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

    WB analysis using 22915-1-AP (same clone as 22915-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22915-1-AP (Caspase 1/P20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 22915-1-AP (same clone as 22915-1-PBS)

    WB analysis of HepG2 using 22915-1-AP (same clone as 22915-1-PBS)

    WB result of Caspase 1/P20 antibody (22915-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 1/p20/p10 transfected HepG2 cells. This data was developed using the same antibody clone with 22915-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Caspase 1/P20 Polyclonal antibody, PBS Only

Caspase 1/P20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22915-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CASP1, Caspase1, CASP 1, CASP-1, Caspase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

22915-1-PBS targets Caspase 1/P20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11143

Product name: Recombinant human Caspase 1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-311 aa of BC062327

Sequence: MADKVLKEKRKLFIRSMGEAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
别名 CASP1, Caspase1, CASP 1, CASP-1, Caspase 1
计算分子量 404 aa, 45 kDa
观测分子量 45-47 kDa, 30 kDa, 35 kDa
GenBank蛋白编号BC062327
基因名称 Caspase 1
Gene ID (NCBI) 834
RRIDAB_2876874
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29466
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize p45 procaspase and the mature fragment p20.

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