验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, K-562 cell, PANC-1 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86423-2-RR targets Casein Kinase 1 Delta in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5856 Product name: Recombinant human CSNK1D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-373 aa of BC003558 Sequence: KTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | casein kinase 1, delta |
| 别名 | casein kinase 1, delta, CKId, CKI-delta, CSNK1D, EC:2.7.11.1 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC003558 |
| 基因名称 | CK1 Delta |
| Gene ID (NCBI) | 1453 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P48730 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.
