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验证数据展示

  • WB analysis using 86791-1-RR (same clone as 86791-1-PBS)

    WB analysis using 86791-1-RR (same clone as 86791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Negative control: mouse liver tissue, mouse brain tissue, mouse skeletal muscle tissue. This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

  • WB analysis using 86791-1-RR (same clone as 86791-1-PBS)

    WB analysis using 86791-1-RR (same clone as 86791-1-PBS)

    Mouse heart tissue were subjected to SDS PAGE followed by western blot with 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 86791-1-RR (same clone as 86791-1-PBS)

    IHC staining of rat heart using 86791-1-RR (same clone as 86791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86791-1-RR (Cardiac Troponin I antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNNI3 antibody included
  • IF Staining of mouse heart using 86791-1-RR (same clone as 86791-1-PBS)

    IF Staining of mouse heart using 86791-1-RR (same clone as 86791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cardiac Troponin I antibody (86791-1-RR, Clone: 251696H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86791-1-PBS in a different storage buffer formulation.

Cardiac Troponin I Recombinant monoclonal antibody, PBS Only

Cardiac Troponin I Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86791-1-PBS

产品说明书

CloneNo. 251696H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

cTnI, TNNI3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.

产品信息

86791-1-PBS targets Cardiac Troponin I in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16365

Product name: Recombinant human TNNI3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-110 aa of BC096165

Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 troponin I type 3 (cardiac)
别名 cTnI, TNNI3
计算分子量 210 aa, 24 kDa
观测分子量 24-26 kDa
GenBank蛋白编号BC096165
基因名称 Cardiac Troponin I
Gene ID (NCBI) 7137
RRIDAB_3745120
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19429
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes.

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