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验证数据展示

  • WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis using 66376-1-Ig (same clone as 66376-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Cardiac Troponin I antibody (66376-1-Ig, Clone: 1D5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNNI3 antibody included
  • IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNNI3 antibody included
  • IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IHC staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66376-1-Ig (Cardiac Troponin I antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNNI3 antibody included
  • WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis of human heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Human heart tissue and skeletal muscle tissue (negative control) were subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    WB analysis of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Mouse heart tissue (6 ug/lane) was subjected to SDS PAGE followed by western blot with 66376-1-Ig (Cardiac Troponin I Antibody) at dilution of 1:5,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    IF Staining of mouse heart using 66376-1-Ig (same clone as 66376-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed mouse heart tissue using 66376-1-Ig(Cardiac Troponin I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66376-1-PBS in a different storage buffer formulation.

Cardiac Troponin I Monoclonal antibody, PBS Only

Cardiac Troponin I Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66376-1-PBS

产品说明书

CloneNo. 1D5D6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Indirect ELISA

别名

cTnI, 1D5D6, TNNI3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.

产品信息

66376-1-PBS targets Cardiac Troponin I in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag16758

Product name: Recombinant human TNNI3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-110 aa of BC096165

Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 troponin I type 3 (cardiac)
别名 cTnI, 1D5D6, TNNI3
计算分子量 210 aa, 24 kDa
观测分子量 26-28 kDa
GenBank蛋白编号BC096165
基因名称 Cardiac Troponin I
Gene ID (NCBI) 7137
RRIDAB_2881753
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP19429
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.

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