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验证数据展示

  • IHC staining of human kidney(renal cell) cancer using 84233-8-RR

    IHC staining of human kidney(renal cell) cancer using 84233-8-RR

    Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CA9 antibody included
  • IHC staining of human kidney(renal cell) cancer using 84233-8-RR

    IHC staining of human kidney(renal cell) cancer using 84233-8-RR

    Immunohistochemical analysis of paraffin-embedded human renal cell cancer slide using 84233-8-RR (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CA9 antibody included
  • Affinity of 84233-8-RR

    Affinity of 84233-8-RR

    Biolayer interferometry (BLl) kinetic assays of 84233-8-RR against Human Carbonic Anhydrase IX/CA9 were performed. The affinity constant is 0.469 nM.

Carbonic Anhydrase IX/CA9 Recombinant monoclonal antibody

Carbonic Anhydrase IX/CA9 Uni-rAb® Recombinant Antibody for IHC, ELISA
Cat No. 84233-8-RR

产品说明书

CloneNo. 241573F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman kidney(renal cell) cancer, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CA9 Ready-To-Use IHC Kit. CA9 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84233-8-RR targets Carbonic Anhydrase IX/CA9 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36941

Product name: Recombinant human CA9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 32-408 aa of BC014950

Sequence: LMPVHPQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carbonic anhydrase IX
别名 CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX
计算分子量 459 aa, 50 kDa
GenBank蛋白编号BC014950
基因名称 CA9
Gene ID (NCBI) 768
RRIDAB_3743534
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16790
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)

实验方案

Product Specific Protocols
IHC protocol for Carbonic Anhydrase IX/CA9 antibody 84233-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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