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验证数据展示

  • IF Staining of adult mouse cerebellum tissue using 82811-1-RR (same clone as 82811-1-PBS)

    IF Staining of adult mouse cerebellum tissue using 82811-1-RR (same clone as 82811-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calretinin antibody (82811-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • WB analysis using 82811-1-RR (same clone as 82811-1-PBS)

    WB analysis using 82811-1-RR (same clone as 82811-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82811-1-RR (Calretinin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IF Staining of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)

    IF Staining of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Calretinin antibody (82811-1-RR, Clone: 2F20 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    IHC staining of mouse brain using 82811-1-RR (same clone as 82811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • FC experiment of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)

    FC experiment of SH-SY5Y using 82811-1-RR (same clone as 82811-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Calretinin Recombinant antibody (82811-1-RR, Clone:2F20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82811-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82811-1-PBS

    Affinity and Kinetic Characterization of 82811-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 82811-1-PBS against Human Calretinin was performed. The affinity constant is 7.60 nM.

Calretinin Recombinant monoclonal antibody, PBS Only

Calretinin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82811-1-PBS

产品说明书

CloneNo. 2F20

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CALB2, 29 kDa calbindin, 2F20, CAB29, CAL2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

产品信息

82811-1-PBS targets Calretinin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag2924

Product name: Recombinant human Calretinin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-271 aa of BC015484

Sequence: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calbindin 2
别名 CALB2, 29 kDa calbindin, 2F20, CAB29, CAL2
计算分子量 29 kDa
观测分子量29 kDa
GenBank蛋白编号BC015484
基因名称 Calretinin
Gene ID (NCBI) 794
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22676
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

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