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验证数据展示

  • WB analysis of PC-3 using 13675-1-AP (same clone as 13675-1-PBS)

    WB analysis of PC-3 using 13675-1-AP (same clone as 13675-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13675-1-AP (Calpain 11 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 13675-1-AP (same clone as 13675-1-PBS)

    IHC staining of mouse testis using 13675-1-AP (same clone as 13675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13675-1-AP (Calpain 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13675-1-AP (same clone as 13675-1-PBS)

    IF Staining of HeLa using 13675-1-AP (same clone as 13675-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpain 11 antibody (13675-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13675-1-PBS in a different storage buffer formulation.

Calpain 11 Polyclonal antibody, PBS Only

Calpain 11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13675-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:3.4.22.-, CAPN11, CANP 11, Calpain-11, calpain11

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13675-1-PBS targets Calpain 11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4668

Product name: Recombinant human CAPN11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 352-702 aa of BC033733

Sequence: YWHTTFYEGSWRRGSSAGGCRNHPGTFWTNPQFKISLPEGDDPEDDAEGNVVACTCLVALMQKNWRHARQQGAQLQTIGFVLYAVPKEFQNIQDVHLKKEFFTKYQDHGFSEIFTNSREVSSQLRLPPGEYIIIPSTFEPHRDADFLLRVFTEKHSESWELDEVNYAEQLQEEKVSEDDMDQDFLHLFKIVAGEGKEIGVYELQRLLNRMAIKFKSFKTKGFGLDACRCMINLMDKDGSGKLGLLEFKILWKKLKKWMDIFRECDQDHSGTLNSYEMRLVIEKAGIKLNNKVMQVLVARYADDDLIIDFDSFISCFLRLKTMFTFFLTMDPKNTGHICLSLEQWLQMTMWG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain 11
别名 EC:3.4.22.-, CAPN11, CANP 11, Calpain-11, calpain11
计算分子量 702 aa, 81 kDa
观测分子量 81 kDa
GenBank蛋白编号BC033733
基因名称 Calpain 11
Gene ID (NCBI) 11131
RRIDAB_2069480
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMQ6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calpains are a superfamily of related proteins, some of which have been shown to function as intracellular calcium-dependent cysteine proteases. Calpains consist of a variable large (80 kDa) subunit and an invariant small (30 kDa) subunit. The expression of calpain11 during spermatogenesis and its localization is spermatozoa suggest that it is involved in regulating calcium-dependent signal transduction events during meiosis and sperm functional processes(PMID:16541461). And the 110 kDa band protein may be regarded as a complex.

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