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验证数据展示

  • WB analysis of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

    WB analysis of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

    IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

    IHC staining of human testis using 12629-1-AP (same clone as 12629-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

    IP experiment of mouse testis using 12629-1-AP (same clone as 12629-1-PBS)

    IP result of anti-Calmegin (IP:12629-1-AP, 4ug; Detection:12629-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12629-1-PBS in a different storage buffer formulation.

Calmegin Polyclonal antibody, PBS Only

Calmegin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12629-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12629-1-PBS targets Calmegin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3322

Product name: Recombinant human CLGN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 311-610 aa of BC028357

Sequence: PAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calmegin
别名
计算分子量 610 aa, 70 kDa
观测分子量 93 kDa
GenBank蛋白编号BC028357
基因名称 Calmegin
Gene ID (NCBI) 1047
RRIDAB_2276364
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14967
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.

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