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验证数据展示

  • WB analysis of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    WB analysis of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    IP experiment of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    IP result of anti-Cadherin-13 (IP:12618-1-AP, 4ug; Detection:12618-1-AP 1:700) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    IHC staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    IHC staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    IF Staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cadherin-13 antibody (12618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    IF Staining of mouse heart using 12618-1-AP (same clone as 12618-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Cadherin-13 antibody (12618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12618-1-PBS in a different storage buffer formulation.

Cadherin-13 Polyclonal antibody, PBS Only

Cadherin-13 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 12618-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

CDH13, Cadherin 13, CDHH, H cadherin, H-cadherin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12618-1-PBS targets Cadherin-13 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3295

Product name: Recombinant human CDH13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 364-713 aa of BC030653

Sequence: TKKEFQATVEEGAVGVIVNLTVEDKDDPATGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTRQEDLSVGSVLLTVNATDPDSLQHQTIRYSVYKDPAGWLNINPINGTVDTTAVLDRESTFVDNSVYTALFLAIDSGNPPATGTGTLLITLEDVNDNAPFIYPTVAEVCDDAKNLSVVILGASDKDLHPNTDPFKFEIHKQAVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCRNSKVDCNAAGALRFSLPSVLLLSLFSLACL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cadherin 13, H-cadherin (heart)
别名 CDH13, Cadherin 13, CDHH, H cadherin, H-cadherin
计算分子量 713 aa, 78 kDa
观测分子量 105 kDa
GenBank蛋白编号BC030653
基因名称 Cadherin-13
Gene ID (NCBI) 1012
RRIDAB_10644130
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55290
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-13 (CDH13, also known as H-cadherin or T-cadherin) is an atypical member of cadherin family, lacking transmembrane and cytosolic domains and anchored to the exterior surface of the plasma membrane via a GPI anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is highly expressed in heart, and in the CNS, CDH13 is expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. It is often down-regulated in cancerous cells. The CDH13 cDNA encodes a 713-amino acid protein, which has a calculated molecular weight of 78 kDa. Studies have detected variable bands on Western blots, including 105 kDa (mature form), 130 kDa (precursor), and 45 kDa, which may be a post-translational processing variant of CDH13 (PMID: 19399994).

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