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验证数据展示

  • IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

    IHC staining of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25390-1-AP (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPECC1L/CYTSA antibody included
  • WB analysis of NIH/3T3 using 25390-1-AP (same clone as 25390-1-PBS)

    WB analysis of NIH/3T3 using 25390-1-AP (same clone as 25390-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

    IP experiment of mouse brain using 25390-1-AP (same clone as 25390-1-PBS)

    IP result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 25390-1-PBS in a different storage buffer formulation.

CYTSA Polyclonal antibody, PBS Only

CYTSA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 25390-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SPECC1L/CYTSA Ready-To-Use IHC Kit. SPECC1L/CYTSA primary antibody included.

产品信息

25390-1-PBS targets CYTSA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21896

Product name: Recombinant human CYTSA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-352 aa of BC136471

Sequence: MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytospin A
别名
计算分子量 1117 aa, 125 kDa
观测分子量 130 kDa
GenBank蛋白编号BC136471
基因名称 CYTSA
Gene ID (NCBI) 23384
RRIDAB_2880056
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ69YQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYTSA, also named as SPECC1L or KIAA0376, is a 1117 amino acid protein, which contains one CH domain and belongs to the cytospin-A family. CYTSA localizes in the cytoplasm and is involved in cytokinesis and spindle organization. CYTSA may play a role in actin cytoskeleton organization and microtubule stabilization, hence it is required for proper cell adhesion and migration.

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