验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16687-1-AP targets Cytohesin 3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10025 Product name: Recombinant human CYTH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-399 aa of BC008191 Sequence: MNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRVVEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytohesin 3 |
| 别名 | CYTH3, ARF nucleotide-binding site opener 3, Cytohesin-3, Grp1, PH, SEC7 and coiled-coil domain-containing protein 3 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC008191 |
| 基因名称 | Cytohesin 3 |
| Gene ID (NCBI) | 9265 |
| RRID | AB_2261396 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43739 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytohesin 3 (CYTH3), also known as GRP1, ARNO3, or PSCD3, is a member of the PSCD family of guanine nucleotide exchange factors (GEFs) that regulate ADP-ribosylation factor (ARF) GTPases. Through its Sec7 domain, CYTH3 facilitates the exchange of GDP for GTP on ARF proteins, thereby modulating vesicle biogenesis, protein sorting, and Golgi apparatus structure and function. Clinicopathological studies demonstrate that CYTH3 is significantly upregulated in hepatocellular carcinoma (HCC) and negatively correlates with patient survival.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytohesin 3 antibody 16687-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
