CYR61 Monoclonal antibody
CYR61 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2E11F11
验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, A431 cells, PC-3 cells, HeLa cells, MDA-MB-231 cells |
Positive IHC detected in | human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells, A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
67656-1-Ig targets CYR61 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Immunogen | CYR61 fusion protein Ag25129 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | cysteine-rich, angiogenic inducer, 61 |
Synonyms | CCN family member 1, CCN1, CYR61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1 |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 40-42 kDa |
GenBank Accession Number | BC001271 |
Gene Symbol | CYR61 |
Gene ID (NCBI) | 3491 |
RRID | AB_2882854 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O00622 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CYR61 antibody 67656-1-Ig | Download protocol |
IHC protocol for CYR61 antibody 67656-1-Ig | Download protocol |
IF protocol for CYR61 antibody 67656-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Hum Cell Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma via regulating YAP activity. | ||
Environ Toxicol Inhibition of Ras protein activator like 2 produces antitumor effects in gastric cancer via the suppression of YAP1 activation |