验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, A431 cells, PC-3 cells, HeLa cells, MDA-MB-231 cells |
| Positive IHC detected in | human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy CYR61 Ready-To-Use IHC Kit. CYR61 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
67656-1-Ig targets CYR61/CCN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25129 Product name: Recombinant human CYR61 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-239 aa of BC001271 Sequence: EDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cysteine-rich, angiogenic inducer, 61 |
| 别名 | CYR61, 2E11F11, CCN family member 1, CCN1, Cellular communication network factor 1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 40-42 kDa |
| GenBank蛋白编号 | BC001271 |
| 基因名称 | CYR61 |
| Gene ID (NCBI) | 3491 |
| RRID | AB_2882854 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00622 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CYR61/CCN1 antibody 67656-1-Ig | Download protocol |
| IHC protocol for CYR61/CCN1 antibody 67656-1-Ig | Download protocol |
| WB protocol for CYR61/CCN1 antibody 67656-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Cell Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma via regulating YAP activity. | ||
Environ Toxicol Inhibition of Ras protein activator like 2 produces antitumor effects in gastric cancer via the suppression of YAP1 activation | ||
Biol Reprod Human trophoblast invasion and migration are mediated by the YAP1-CCN1 pathway: defective signaling in trophoblasts during early-onset severe preeclampsia | ||
J Orthop Translat Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis | ||
Mech Ageing Dev Cyr61 Promotes D-gal-induced Aging C2C12 Cell Fibrosis by Modulating Wnt/β-catenin Signaling Pathways |
