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验证数据展示

  • WB analysis using 82927-1-RR (same clone as 82927-1-PBS)

    WB analysis using 82927-1-RR (same clone as 82927-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82927-1-RR (CYP7B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82927-1-RR (same clone as 82927-1-PBS)

    IF Staining of HepG2 using 82927-1-RR (same clone as 82927-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82927-1-RR (same clone as 82927-1-PBS)

    FC experiment of HeLa using 82927-1-RR (same clone as 82927-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYP7B1 Recombinant antibody (82927-1-RR, Clone:230160H5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82927-1-PBS in a different storage buffer formulation.

CYP7B1 Recombinant monoclonal antibody, PBS Only

CYP7B1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82927-1-PBS

产品说明书

CloneNo. 230160H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

230160H5, 24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CBAS3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CYP7B1 Ready-To-Use IHC Kit. CYP7B1 primary antibody included.

产品信息

82927-1-PBS targets CYP7B1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19374

Product name: Recombinant human CYP7B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 96-403 aa of BC136574

Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome P450, family 7, subfamily B, polypeptide 1
别名 230160H5, 24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CBAS3
计算分子量 506 aa, 58 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC136574
基因名称 CYP7B1
Gene ID (NCBI) 9420
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75881
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

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