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CYP51A1 Polyclonal antibody

CYP51A1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13431-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IP, ELISA and More (2)

Cytochrome P450 51A1, Cytochrome P450 14DM, CYPLI, CYPL1, CYP51

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, rat liver tissue, HuH-7 cells
Positive IP detected inmouse testis tissue, mouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13431-1-AP targets CYP51A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, chicken, hamster
免疫原 CYP51A1 fusion protein Ag4235 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 51, subfamily A, polypeptide 1
别名 Cytochrome P450 51A1, Cytochrome P450 14DM, CYPLI, CYPL1, CYP51
计算分子量 509 aa, 57 kDa
观测分子量 55 kDa
GenBank蛋白编号BC032322
基因名称 CYP51A1
Gene ID (NCBI) 1595
RRIDAB_2088571
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16850
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).

实验方案

Product Specific Protocols
WB protocol for CYP51A1 antibody 13431-1-APDownload protocol
IF protocol for CYP51A1 antibody 13431-1-APDownload protocol
IP protocol for CYP51A1 antibody 13431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
mouseWB

Nat Commun

Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions

Authors - Shengjie Fan
humanWB

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex

Authors - Michael L van de Weijer
humanWB

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Authors - Michael L van de Weijer
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
human,mouseWB

Nat Commun .

MMAB promotes negative feedback control of cholesterol homeostasis

Authors - Leigh Goedeke
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