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验证数据展示

  • IHC staining of human skin using 20011-1-AP

    IHC staining of human skin using 20011-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 20011-1-AP

    IHC staining of human skin using 20011-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20011-1-AP (CYP4F8 Antibody) at dilution of 1:50 (under 40x lens).

CYP4F8 Polyclonal antibody

CYP4F8 Polyclonal Antibody for IHC, ELISA
Cat No. 20011-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

CPF8, CYP4F8, CYPIVF8, Cytochrome P450 4F8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman skin tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20011-1-AP targets CYP4F8 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 4, subfamily F, polypeptide 8
别名 CPF8, CYP4F8, CYPIVF8, Cytochrome P450 4F8
计算分子量 60 kDa
GenBank蛋白编号NM_007253
基因名称 CYP4F8
Gene ID (NCBI) 11283
RRIDAB_2878634
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP98187
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP4F8, also named as CPF8, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. CYP4F8 hydroxylates arachidonic acid (20:4n-6) to (18R)-hydroxyarachidonate. CYP4F8 shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. CYP4F8 catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3-hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). It catalyzes hydrxylation of PGI2 and carbaprostacyclin. The antibody has no cross reaction to other CYP4F member

实验方案

Product Specific Protocols
IHC protocol for CYP4F8 antibody 20011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols